Evaluation acute toxicity and antibacterial activity of Penicillium sp endophitic fungus extract of kunyit putih (Curcuma zedoaria) in mice (Mus musculus L.) had been done. Acute toxicity at concentration until 2000 mg/kg BW and anticahterial test was done used S. dysentriae bacteria by giving 6 treatments contain treatment I (normal control), treatment II (negative control), treatment III (dose I 125 mg/kg BW), treatment IV (dose 250 mg/kg BW), treatment V (dose 500 mg/kgBB), and treatment VI ( positive control ciprofloxacin dose 10 mg/kg BW). Observation was done based on amount of S. dycentriae bacteria in faeces taken once in 2 days along 12 days. Bacteria population data on the mice faeces analyzed by statistical analysis. At acute toxicity no mortality was observed at 2000 mg/kg BW for 24 h observation. Observed for toxic symptoms such as behavioural change, locomotion convulsion was not found. The experiment antibachterial activity result that treatment III to VI show the population of Shigella which is not different. Effect treatment time to population of bacteria shows in the 10 and 12 the bacteria amount fall below infection dose (2,40 x 105 and 6,76 x 104 ) respectively, thus can be concluded that the extract had acted as a cure.