Physico-chemical and microbiological parameters wer e analyzed in leachate affected groundwater samples obtained at different locations viz. Bhanpor Distri ct, Union Carbide Area, Shivnagar, Mohali village, Peoples Group Colony and Nishatpora. during December, 2008 to May 2009 in order to assess the impact of leacha te percolation on groundwater quality in District, Bho pal (M.P). Different physico-chemical parameters (p H, Total Dissolved Solids, Electrical Conductivity, Dissolve d Oxygen, Free Carbon dioxide, Alkalinity, Chloride , Total Hardness and Biological Oxygen Demand) and bacterio logical parameters (Total Coli form, Fecal Coli for ms and Fecal Streptococci) were analyzed using standard me thodology in each of the collected samples. Total d issolved solids (TDS), electrical conductivity (EC), and Na + exceeded the World Health Organization (WHO) toler ance levels for drinking water in 62.5%, 100%, and 37.5% of the groundwater samples respectively with pH an d Fe exceeding WHO limits in 75% of the samples. Our res ults suggest the altered Physico-chemical and bacte riological parameters compared to the drinking water standards laid by Indian Council of Medical Research (ICMR) and World Health Organization (WHO). For example the el ectrical conductance was in the range of 980 – 1490 simens. A good number of samples are also showing significa nt amount of organic matter that provides nutrition for the growth and multiplication of microorganisms, thus n ot fit for drinking purpose. Thus it was concluded that most of the water samples were non-portable for human being s due to high concentration of one or the other par ameter and some remedial measures are suggested to reduce furt her groundwater contamination via Leachate percolat ion, hence the present study demands for proper manageme nt of wastes in Bhopal.