Biosorption process has been proposed as an efficient, potential, cost effective way of removing toxic metals from industrial effluents at low concentrations. In the present study a new biosorbent material, agro waste Psidium guajava leaves powder was used as an adsorbent. Studies on the removal of Chromium from aqueous solutions using Psidium guajava leaves powder were undertaken. The effect of metal concentration, pH of aqueous metal solution, temperature and weight of biomass were studied. The results indicate that the amount of Chromium adsorbed increased with increase in metal concentration, weight of biomass and pH of the aqueous phase. Finally an experimental equation was developed to estimate the equilibrium distribution of Chromium between Guava leaf biomass and aqueous metal solution incorporating various parameters studied.