Although Glycyrrhiza glabra is the recognized source of glycyrrhizin, its production in tissue culture has not been successful by tissue culture. Abrus precatorius is an alternative source for glycyrrhizin and was thus taken up in tissue culture technique for glycyrrhizin production. The yield enhancement for the glycyrrhizin production was done by means of genetic transformation using Agrobacterium tumefaciens. When compared with untransformed callus resulted in 8.665 ± 0.144 g/l dry cell weight biomass and 0.427 ± 0.0016% glycyrrhizin, which was 2.4-fold times higher in productivity in comparison to control untransformed cultures.