Endophytic fungi isolated from sambiloto (Andographis paniculata), were investigated for fungal lipid production as a biodiesel precursors. The fungi Aspergillus sp1 (SbD3), Aspergillus sp2 (SbD5), Aspergillus sp3 (SbD6), and Penicillium sp (SbD4) were cultured in flasks with cultivated in Potato Dextrose Broth (PDB) medium for 8 days in static. Dried fungal biomass (30 g) were subjected to addition of each solvent in chloroform, methanol, water (1:2:1 (v/v/v). The reaction mixture was left to reflux for 24 h. After that period, the reaction mixture was filtered and concentrated in a rotatory evaporator. The lipid compounds were then extracted by liquid-liquid extraction using nhexane, and concentrated in a rotatory evaporator. The fungal lipid were determined on the basis of spectroscopic analysis (FTIR and 1H-NMR). In conclusion, this work revealed the possibility of using the promising Aspergillus sp2 (SbD5) in biodiesel production.