In human physical conditions, the interaction of topiramate amyl cephalosporin cefotaxime (TACC) with bovine serum albumin (BSA) and the effects of coexistent drugs and metal ions were investigated by fluorescence spectroscopy. Results showed that the effect between TACC and BSA was static fluorescence quenching process, and the electrostatic attraction plays an important role in the conjugation reaction of BSA and TACC. Coexistent contents such as ampicillin, gentamicin, streptomycin, neomycin, kanamycin, thiamphenicol, erythromycin, acetyl spiramycin, sulfamethoxazole, penicillin G sodium salt, cefotaxime sodium, cefoperazone sodium, as well as Zn2+、 Co2+、Ni2+、Fe3+ and Mg2+ have no effects on the binding of BSA-TACC system, but a bit of Cu2+, moxifloxacin, ciprofloxacin, norfloxacin, chloramphenicol could disturb the stability of BSA-TACC system, making the fluorescence intensity changed a lot.