Both monomers 8-quinolinyl methacrylate (8-QMA) and 2-(N-phthalimido) ethyl acrylate (NPEA) are biologically active and their possible applications are in the field of the biomedical and membrane science. In this study, copolymers of NPEA and 8-QMA of various compositions were prepared using free radical solution polymerization technique. The composition of the copolymers was determined by Ultra-Violet (UV) spectroscopy. The reactivity ratios of the monomers were calculated using the conventional linear methods. The value r2 is slightly higher than r1 which suggest that monomeric units of NPEA and 8-QMA in copolymers were negligibly varied. Gel permeation chromatography is employed to evaluate the average molecular weights of the copolymers. Thermal stability of the copolymers was confirmed from the thermogravimetric analysis (TGA). To confirm antimicrobial properties of copolymers, they are assessed on various microorganisms. It is found that as increases of 8-QMA content in the copolymers, copolymers were more effective on growth of microorganisms.