The increasing of antibiotic resistance is the serious problem of most pathogenic bacteria including Helicobacter pylori, which is the etiologic agent of chronic gastritis, peptic ulceration, gastric carcinoma and gastric MALTlymphoma. An alternative treatment using traditional plants is advantageous in less or nontoxic, cheap and availability in rural areas. In this study, we examined the activities of 19 Thai fruit mesocarp extracts on growth of H. pylori by agar dilution method and anti-adhesion activities of H. pylori ATCC43504 against HEp-2 cells by spectrofluorometry. The ethanolic extract of sugar apple (Annona squamosa L.) showed the most potent with MIC50 of 20 mg/ml and superior anti-adhesion activity of 76.3% against HEp-2 cells. The potential anti-H. pylori activities were observed from ethanolic extracts of mangosteen, durian, jujube, papaya, rambutan juice, mango juice. While, potential anti-adhesion activities were derived from ethanolic extracts of mangosteen, mango, sapodilla, rose apple and both ethanolic and aqueous extracts of guava. Although, pharmacological activities of mesocarp of fruits were lower than exocarp (peel), endocarp (seed), leaf and other parts, it is the consumable part that people could gain pharmacological substances directly apart from great flavor.