Nitazoxanide, a thiazolidine compounds, is an efficient, broad-spectrum anti-parasitic and anti-microbial drug. On the one hand, by measuring breathing rate, breathing extent and electrocardiogram, effect of nitazoxanide on cardiovascular system and respiratory system of anesthetized rats was tested. On the other hand, by observing the activities and behavior change of mice, conducting climbing pole test and muscle fatigue test, the effect of nitazoxanide on nervous system was obtained. Results indicated that in the dose range 100mg-400mg/kg, nitazoxanide affected little to cardiovascular system and respiratory system of anesthetized rats and did not significantly inhibit central nervous system of mice.