Melittin (MEL) is the main toxin protein constituent of bee venom isolated from A cerana with powerful antiproliferation activity. This research aimed to evaluation effect of melittin isolated from A. cerana on antiproliferation in human cancer cervix Hela cells through activation of p53 and caspase 3 protein using ICC method. The result show that melittin induced expression of p53 and caspase 3 as apoptotic marker on cell on HeLa cell line (p<0.05) and has a potential as candidate of anticancer drug in future.