This research aimed to investigate the effect of drying period and harvesting dates at different plant parts on vegetative herb, volatile oil content and its composition of Melissa officinalis harvested 8 cuts. Increasing of the drying times led to decreased of herb, leaves and stem weights in all cuttings and 4th cut yielded the highest weights of herb, leaves and stem in all drying periods (0, 5, 10 and 20 days). However, the highest weights were obtained from fresh herb (207.1-190.0g/plant; 16.6-15.3 ton/fed.), fresh leaves (117.1-110.6g/plant; 9.3-8.05 ton/fed.) and fresh stem (90.1-90.4 g/plant; 7.2-7.2 ton/fed.) in 4th cut in the first and second seasons, respectively. Essential oil percentage of Melissa herb and leaves increased by the drying process, where it reached the highest % in plants dried for a period of 5 days followed by drying period of 10 days and then drying for 20 days. Stems contain neglectable amount of essential oil compared to herb and leaves and failed in many cuttings and completely disappeared by drying particularly at 20 days in all cuttings. In general, plants harvested in 1st cut and dried for 5 days gave the highest essential oil % (0.307-0.32 % in herb and 0.39-0.40% in leaves) and oil yield in herb (10.2- 10.8 l/fed.) as well as fresh leaves in 1st cut gave the highest oil yield (9.63-10.08 l/fed.) followed by 7th cut compared to the rest of cuttings in the first and second seasons, respectively. Plant parts, drying process and cut dates have an impact on the geranial and neral contents. The highest contents of neral and geranial were obtained from leaves, herb and stem. Increasing drying period decreased geranial and neral contents. Plants harvested in 1st cut gave the highest neral and geraniol contents.