Bioethanol can be used as a second generation advanced biofuels. Currently it is mainly produced from starch but bioethanol production from starch leads to competition for food, land and price. Therefore, ligno-cellulosic agricultural residues are potentially used for bioethanol production to solve such challenges. The efficiency of the fermentation process mainly depends on the amount of reducing sugars which is further enhanced by selecting an efficient pretreatment process. In the present work Tamarind seeds have been chosen as the substrate. The yield of bioethanol mainly depends on the yield of reducing sugars which is again dependent on the various pretreatment methods used. So, the proposed work aims to carry out different pretreatment methods to identify the best pretreatment method for enhancing the yield of reducing sugars. The tamarind kernel powder will be extracted from tamarind seeds and the extracted tamarind kernel powder is subjected to various pretreatment methods like acid pretreatment, alkaline pretreatment and steam explosion. The amount of reducing sugars obtained, were then determined by di-nitro salicylic acid method. It was found that acid pretreatment with 0.3N HCl and 0.3N H2SO4 is the best pretreatment method among the selected pretreatment methods