Urinary citrate appears to be an important factor in the crystallization process of calcium oxalate and calcium phosphate. Oxaluria can be reduced by means of reducing intestinal absorption of oxalate through feeding a mixture of freeze-dried lactic acid bacteria. Chelation therapy is now a days expanding its applications in various treatments. Calcium chelation by citric acid and lactic acid can play a major role in chelation therapy of naphrolithiasis. So, in present study, we analyzed the effect and prospect of chelating agents in urological calcium oxalate calculi dissolution as around 70% calculi are of calcium oxalate type. Present study concluded that as the concentration of citric acid and lactic acid increases the calcium ion concentration in soluble form increases. Statistical analysis of data with F test shows calculated F value is larger than tabulated F, a significant difference in response to dissolution with citric acid and lactic acid at df =1 between sample and df = 10 within samples, p = 0.05