Heavy metals are the metals having a density at least five times more than that of water. The effects of different concentration (1, 2, 3, 4 and 5 mg/l) of arsenic, manganese and chromium on germination, root growth and protein content of green gram (Vigna radiata. L) and black gram (Vigna mungo L.) were studied. The inhibition of germination, root growth and protein content was noticed at higher concentrations of Arsenic, manganese and chromium. The results also indicated that the germination percentage, root length and protein content were gradually decreased with the increasing concentration of both heavy metals.