Fish are permanently exposed to different external hazards because of their intimate contact with aquatic environments. The bacterial infection is an important and limiting factor of intensive fish production because they have shown tight connection with various body functions of host i.e., metabolism, immunity, energy utilization and health maintenance. Proteus vulgaris is an opportunistic pathogen of fish because it is the most common bacterial species of inland water and sediments. It causes gastrointestinal and non-gastrointestinal disease i.e. hemolytic syndrome, kidney disease, ulcerative disease, urinary tract infection (UTI) etc.in various teleost. Proteus vulgaris acknowledge their presence in many catfish, which were collected from fish market of and around Jalandhar district during spring season. Confirmation came after biochemical test performed in university lab and specific test through BD Phoenix machine done at ICAR Research Complex (Meghalaya). It has been found that isolated colonies were susceptible to azithromycin, kanamycin, chloramphenicol and ciprofloxacin. Against neomycin and gentamycin 72% colonies were susceptible and rest 28% shows partial growth. About 86% of colonies were susceptible to ofloxacin. But important finding of this work is that, colonies are resistant against drugs like amoxicillin, penicillin, ampicillin and methicillin. But with ofloxacin, gentamycin and neomycin more study is to be carried out.