Two of copper(II)complexes,bis(1-methylamidino-O-methylurea)copper(II)chloride(1) and bis(1- ethylamidino-O-methyl urea)copper(II)chloride(2)were prepared. The complexes were characterized by analytical analysis, IR and UV-Vis spectroscopy. The interaction of calf thymus DNA with copper(II) complex has been investigated by using the absorption titration method and thermal denaturation method. The experimental results showed that the mode of binding is nonintercalative. The synthesized complexes were also screened for their antibacterial activity against isolated strains of Escherichia coli, Klebsiellapneumoniae and Proteus mirabilisby using disc diffusion method. Both the complexes inhibits the bacterial growth.