Leuconostoc mesenteoides is commonly used in fermented food. The study on developing molecularly targeted drugs to achieve a higher grade of drug delivery system that is one of the indispensable issues in pharmaceutical fields. This paper reported the effects of carbon sources including in glucose, maltose, lactose, saccharose at the different concentrations as 0, 5, 10, 20, 30 g/L on the cell differentiation of Leuconostoc mesenteroides VTCC-B-871. As results, L. mesenteoides VTCC-B-871 formed minicells with highly significant number of 4.6 ± 0.3 (%) starting cells in modified MRS broth with 20 % glucose. The minicells were collected and checked for the less than 400 nm in size and round shape under scanning electron microscope. Therefore, minicells could be used as a nanoparticle in pharmaceutical science.