This study presents the report of TLC densitometric method, which has been developed and validated for quantification of Oleanolic acid from hydroalcoholic extract of bark of Madhuca longifolia. The separation was performed on TLC aluminum plates precoated with silica gel 60 F254. Good separation was achieved in mobile phase using Toluene: acetic acid: formic acid (9:4:0.5). Determination and quantitation were performed by densitometric scanning at 560 nm in reflection/ absorbance mode. This method gave compact spots at Rf 0.53 corresponding to oleanolic acid. The method was validated using ICH guidelines in terms of precision, repeatability and accuracy. Linearity range for oleanolic acid was 100–600 ng/spot with correlation coefficient R2 ±SD = 0.990 ±5.05% in the concentration range 100–600 ng/spot. The LOD and LOQ were found to be 9.302 and 28.1 ng, respectively. The content in crude extract obtained from bark was found to be 0.418± 0.005% w/w. HPTLC method was found to be simple, precise, accurate and convenient method for rapid screening of active constituents present in hydro-alcoholic extract of plant and can be used for analysis and routine quality control of herbal material and formulations containing Madhuca longifolia.