A simple, isocratic, rapid and sensitive high performance liquid chromatographic method has been developed for quantitative determination of Trazodone Hydrochloride and its three process related impurities. The method has been validated for the determination of related substances in Trazodone Hydrochloride using a C18, ODS (4.6mm×250mm×10µm) column by keeping the flow rate of 1.5ml / min and having sensitivity of 0.2. The elution is carried out using a mobile phase consisting of methanol l80 ml, Acetonitrile 180 ml, Tetrahydrofuran 40 ml, and Trifluroacetic acid (0.5%) 600 ml. The detection is been carried out at 252 nm with injection volume of 10 µL. The run time is 15 minutes for estimation of related substance. The precision, Linearity and accuracy of the method are demonstrated for Trazodone Hydrochloride. Specificity of the method is also been studied. Limit of Detection and Limit of Quantification is also carried out. The method is found to be stability indicating and useful for the analysis of related substances of Trazodone Hydrochloride .Impurity profiling is also been carried out.