A new simple, specific, sensitive and cost effective UV-spectrophotometric method has been developed for the estimation of Cefixime in pharmaceutical dosage form.Wavelength selected for estimation of Cefixime was 290.60 nm. The developed method was validated statistically with respect to precision, accuracy, specificity, linearity and range. Linearity was studied over concentration range of 2-40 μg /ml and correlation coefficient was found to be 0.9997 for regression line. The method described can be successfully employed for assay of Cefixime in bulk drug and tablet dosage form.