ICP-OES and 5-Br-PADAP spectrophotometry are commonly used in determination of In3+ in solution. In this study, the linear range, detection limit, precision, recovery and the application effects of ICP-OES and 5-Br-PADAP spectrophotometry on determination of In3+ were investigated. The results showed that The results showed that the detection operation of ICP-OES was easier and more convenient in comparison with 5-Br-PADAP. By ICP-OES analysis, wide detection linear range and low the detection limit were obtained. In addition, the detection RSD value of ICP-OES was smaller, meaning the good agreement between determined values and certified values. As a result, ICP-OES was more suitable for determination of In3+ in solution than 5-Br-PADAP spectrophotometry.