Communities are concerned with the role of durable pesticides and their carry over effect in food chains. Accordingly, the recognition and measurement of chemical compounds in food, environment and living bodies is necessary. In this study, which dated from October 2010 to March 2011, deltamethrin toxin residues in sugar beets were investigated in the cities of Isfahan province including: Isfahan, Borkhar, Semirom, Golpayegan and Fereidan using high performance thin layer chromatography (HPTLC). Maximum amount of deltamethrin belonged to Borkhar region and the minimum amount was attributed to two regions of Isfahan and Golpayegan. The toxin residues in sugar beets in all investigated areas were higher than the maximum allowable residues of any pesticide MRL (0.01 mg in kg sugar).