Macrolides have been known for >5 decades.Macrolide-lincosamide-streptogramin (MLS) antibiotics are commonly used in treatment of staphylococcal infections. Clindamycin is also used as an alternative for patients who are allergic to penicillin. In the present study Staphylococcus aureus isolates was done to find out the percentage of Staphylococcus aureus having inducible clindamycin resistance in our geographic area by using Dtest. Various clinical samples like pus, urine, stool, sputum, blood and other body fluids of patients attending Shri B M Patil Medical College and Hospital were selected for study from January 2013 to June 2013. Samples which yielded Staphylococcus aureus were included in the study. S. aureus was identified by conventional techniques. Isolates were screened for erythromycin resistance. The isolates that were found to be erythromycin resistant were further tested for inducible clindamycin resistance by using D- Test. A total of 51 consecutive, non duplicate isolates of Staphylococcus aureus were recovered from various clinical specimens in the Department of Microbiology during the study period. The present study showed higher rates of iMLSB(20%) when compared with cMLSB (10%)phenotypes. The present study showed higher rates of iMLSB(20%) when compared with cMLSB (10%)phenotypes. Therefore we recommend the use of D test for detecting the inducible clindamycin resistance ,so that the misuse of clindamycin is prevented and also limiting the treatment failure in many patients suffering from serious infections.