Fourier Transform Infrared Spectroscopy is one of the most modern techniques used in the field of analytical chemistry for the identification of the constituents of the substance by determination of the functional groups. In Forensic Science the IR spectroscopy is very helpful in the identification of the substances by knowing their constituents. Along with the determination of active components of ghee, the trans fatty acids by FT-IR spectroscopy, the adulteration in ghee can also be detected by studying the spectrogram.