The present study was carried out to develop an oral colon targeted drug delivery system, which consists of Theophylline matrix tablets prepared using guar gum and borax cross linked guar gum as rate controlling polymers in different concentrations. These tablets were evaluated for weight variation, friability, hardness uniformity of content and in vitro drug release under specified conditions. The dissolution data revealed that the tablets containing guar gum and borax cross linked guar gum in higher concentrations each (120mg) showed 87.567±0.42% and 76.186±0.17% of drug release respectively. And selected tablets of borax cross linked guar gum were subjected to in vitro drug release study in presence of rat caecal content medium. Results clearly indicate that there is an increase in the release of the drug to 98.930±0.38%.