Wound healing is part of the regeneration of skin tissue from the damage. This will be hampered by the Methicillin resistant Staphylococcus aureus (MRSA) infection. This study aims to determine the effect of +dalethyne against epithelialization in wound healing of the skin MRSA-infected rats. Thirty six Wistar rats, 3 months old, are divided into 6 groups, 2 groups of negative control, 2 groups of positive control, 2 groups of treatment group (All are sacrificed on the fourth day and sixth day). Incision to the back skin of rats by a knife along the incised 2 cm and depth to subcutaneous was done. Wound of positive control groups infected by MRSA, wound in the treatment groups also infected MRSA and then applied topically +dalethyne. Each groups are sacrificed on day 4 and 6, the skin tissue is fixed, made histological preparations, stained with HE. The measurement of the epithelial length using Optilab mounted on a light microscope. The data are analyzed by comparing the mean and SD. The epithelial length in the treatment group was higher than the positive control {(0,46±0,19)vs(0,21±0,16);(0,63±0,76)vs(0,42±0,301), being compared with the negative control is not much different. Conclusion: Topical +dalethyne accelerates epithelialization in wound healing of the skin MRSA-infected rat.