The study was aimed to evaluate the anticancer activity of four Makassarese medicinal plant: Boehmeriavirgata(Forst), Acanthus ilicifoliusLinn, Eupatorium odoratum and AcalyphaindicaL, on human cervical cell lines (HeLa) and macrophage cells and its selectivities. The cytotoxic activity was evaluated by MTT method. IC50 on HeLa cell line wereBoehmeriavirgata (Forst) >Acanthus ilicifolius Linn >Acalyphaindica L. >Eupatorium odoratum and on macrophage cells are Acalyphaindica L. >Boehmeriavirgata (Forst) >Eupatorium odoratum>Acanthus ilicifolius Linn (9.40, 32.81, 179.02 and 223.64 μg/mL) respectively, while Doxorubicin was 1.053 μg/mL. But only Boehmeriavirgata(Forst) and Acanthus ilicifoliusLinn selective as anticancer (SI were 3.10 and 3.14) and have potential to be developed as an anticancer agent.