Curcuma heyneana (Zingiberaceae) is one of the zingiberaceous plants indigenous to Java Island, Indonesia which commonly known as Temu Giring. The rhizome of this plant is considered to be useful for treatments of skin diseases, abrasions, and injuries due to the antibacterial compounds contained in this plant. However, there is still scarce information regarding antibacterial compounds contained in C. heyneana rhizomes. In searching of antibacterial compound, isolation of secondary metabolites from Indonesian C. heyneana had been conducted. Curcumenol had been successfully isolated from methanol extract of C. heyneana rhizomes using extraction and various chromatography techniques. This compound had been elucidated using FTIR, GCMS, and NMR (1D and 2D). Curcumenol showed weak antibacterial activity in 50 ppm with inhibition zone of 4 mm and inhibition index of 0.67 towards Escherichia coli (Gram negative) while this compound was inactive towards Staphylococcus aureus (Gram positive).