Hyaluronic acid and human-like collagen, both with favorable biocompatibility and biodegradability, were designed as biomaterials with excellent property in tissue engineering. We report an injectable hydrogel particle based on hyaluronic acid and human-like collagen. They were crosslinked with divinyl sulfone to form a three- dimensional network. The divinyl sulfone was removed by dialysis and the content was determined by gas chromatography. The in vitro degradation proved an admirable ability of antienzyme. The cytotoxicity analysis showed the low toxicity to the cell growth. The hydrogel is promised to be applied into medical field.