Copper phosphate nanoparticles were synthesized by simple precipitation method. The crystalline nature of the sample was confirmed by X-ray diffraction (XRD). FT-IR establishes the formation of the copper phosphate. The DRS-UV spectrum showed bands corresponding to tetragonal distortion in the coordination sphere around Cu2+. The morphology (layered flakes) and size (50 to 55 nm) was verified by TEM analysis. Copper phosphate nanoparticles were screened for antimicrobial activities against two gram positive bacteria and two gram negative bacteria. The bioactive assay showed that copper phosphate exhibited moderate to good antimicrobial activities against gram positive - staphylococcus aureus (SA), bacillus cereus (BA) and gram negative - E.coli (BB), salmonella (BC) bacteria.