A strain characterized as Bacillus subtilis isolated from soil was used to convertfurfural residue (FR) to biofertilizerbysolid-state fermentation (SSF). Medium for the SSF fermentation was composed of 5% glucose, 2.5% soybean meal, 2.5% rice husk as nutrients and 90% grounddriedFR as structure material.The test organisms increased to 2.32×1010 CFU/g in the medium after 6 days fermentation. Serial dilution plate and PCR-DGGE method were used to investigate the soil microflora. The application of biofertilizersignificantly (p < 0.05) improved the growth of spinach and controlled fungiin soil compared to control.