In recent times, focus on plant research has increased all over the world and a large body of evidence has collected to show immense potential of medicinal plants used in various traditional systems. Use of plant based drugs and chemicals for curing various ailments and personal adornment is as old as human civilization. The goal of research work is to estimate primary metabolites such as protein, lipid, starch, phenol, and carbohydrates in different plant parts which are present in different proportion in plant species viz. Clitoria ternatea, Guazuma ulmifolia, Madhuca indica. The highest amount of soluble sugar was observed 129.0 mg/gdw, protein 67.0 mg/gw in leaf of G. ulmifolia, phenol 46.0 mg/gdw in root of M. indica, lipid 42.0 mg/gdw in root of G. ulmifolia and starch 54.0 mg/gdw in stem of M. indica, similarly lowest amount of sugar was observed 102 mg/gdw, protein 21.0 mg/gdw in root of C. ternatea, and phenol 18.0 mg/gdw in leaf of C. ternatea, lipid 10.0 mg/gdw in leaf of G. ulmifolia and starch 23.0 mg/gdw in leaf of M. indica.