The present study focused on the comparative studies on eco-friendly biogas production from natural waste materials. The biogas production was carried out from animal, kitchen and trees waste by anaerobic digestion process. Batch experimental studies were performed by varying the nature, amount of waste material, temperature, time and pH. It was noticed that the highest amount of biogas was produced from animal waste compared to kitchen and trees waste. The flame test was also conducted to test the biogas production from various type of waste material. As a result, animal waste showed the highest volume of gas and strong flame compares to kitchen and trees waste. Thus, the production of gas has more quality than normal gas and has the potential to use especially agricultural and animal’s origin countries.