In the few decades, there has been exponential growth in the field of herbal medicines. Most of the traditional system of medicine is effective but they lack standardization. So there is a need to develop a standardization technique. Standardization of herbal formulation is essential in order to assess the quality, purity, safety and efficacy of the drug. The present paper reports the investigation and standardization of trikatu churna, an Ayurvedic formulation. Two marketed formulation samples and in-house preparation were subjected to organoleptic study, physical characteristics and physicochemical screening. Trikatu is a compound churna as it consists of more than one ingredient. It consists of coarse powder (sieve 40 size) of fruits of Piper longum, Piper nigrum and rhizomes of Zingiber officinale. The principle of using churnas is due to the fact that therapeutic value of most of the substances greatly increases when they are reduced to a very fine state of subdivision. They are also easily administrable especially in the cases of children where they cannot swallow pills, tablets or capsules. The results of various parameters obtained from the study showed that marketed formulation 2 & In-house have comparable physical values. The flowability of formulation was found to be poor in all formulation. There is no uniformity in preparation of formulations, and these results could be utilized as a reference for setting limits for the reference standards for the quality control and quality assurance of these drugs.