Solanum trilobatum is indigenous medicinal plants most commonly occur in Asia-pacific region. Main aim of this study is to investigate presence of phytochemical such as alkaloids, phenol, glycosides, saponins, phytosterols, flavonoids, tannins, steroids, and terepenoids and antibacterial and antifungal potential of methanolic extract of both leaf and bark of Solanum trilobatum by using four different bacterial pathogens and two different fungal pathogens by agar-well diffusion assay. In antibacterial activity, Methanolic extract of bark showed maximum antibacterial activity for Staphylococcus aureus - MTCC 737 (3.7 mm of zone of inhibition). Similarly, 3.3 mm zone of inhibition against Pseudomonas aeruginosa - MTCC 424. In case of Leaf it shows, 1.7 mm and 2 mm of zone of inhibition respectively. In antifungal activity, leaf shows good activity when compared with methanolic extract of bark. Leaf extracts of Solanum trilobatum shows antifungal activity against Aspergillus brasiliensis – MTCC1344 (2 mm), Aspergillus fumigatus – MTCC 343 (2.7 mm) zone of inhibition. Thus the above studies proved that, clinical importance of bioactive compound from Solanum trilobatum. In future, we can go for further purification and characterization biological active compound from these kinds of medicinal plants.