Hydro-alcoholic leaf extract of three medicinal plants viz., Andrographis paniculata, Cassia alata and Morinda citrifolia were prepared by soxhlet extraction method. The antibacterial activity of hydro-alcoholic leaf extracts were carried out in-vitro using disc diffusion method against six different bacterial strains viz., gram positive (Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis) and gram negative (Escherichia coli, Serratia marcescens and Proteus vulgaris). The antibiotic potency of leaf extract was compared with the standard antibiotics, Rifamycin and Amoxyclav. Leaf extract of Andrographis paniculata was recorded with remarkable inhibition ability against the tested organisms and it was followed by Cassia alata and Morinda citrifolia. Rifamycin was found to be good drug in controlling the pathogens in comparison to Amoxyclav in our study. Pseudomonas aeruginosa didn’t respond to any extracts or the antibiotics and become resistant to all at 15 μl dilution of the extract. Amoxyclav was recorded with low responsive to prevent the growth of the bacteria studied herewith.