Chitinases has an extensive range of applications currently in bio control, medical field, and degradation of pollutant, SCP production, bio pesticides and protoplast isolation. Chitinases are produced by diverse organisms like bacteria, fungi, plants, insects, humans and animals. Different processes like monoculture, co-culture, recombinant cells and immobilized cells are used for the enriched chitinase production. Recombinant strains and immobilized cells are used at the present time for enhanced production of chitinases. Here, we review the different sources, methods, and different factors responsible for chitinase production along with its applications.