This research aimed to find the chemical profile and compared the caffeine content of Arabica coffee (Coffea arabica L.) and coffe in the market. To obtained the chemical profile and the determination of the extract caffeine content of Arabica coffee bean (Coffea arabica L.) by HPTLC method. The results showed that etanol extract of Arabica coffee (Coffea arabica L.) contain caffeine with total content of 120.12 μg/ml and the samples of coffee in the market, namely Coffee A 137.95 μg/ml; Coffee B 262.74 μg/ml; Coffee C 304.65 μg/ml; Coffee D 202,53 μg/ml and Coffee E 24.08 μg/ml. Then the highest total caffeine content was obtained in coffee C as much as 304.65 μg/ml.