The seed oil of the highly promoted food plant Moringa oleifera Lam was extracted by solvent extraction using Soxhlet apparatus. The oil was methylated and analyzed on a Shimadzu GC-MS QP2010 SE.The oil was also screened against local strains of Mycobacterium tuberculosis by tetrazolium dye microbroth dilution assay and was found to be active at 25% (v/v). The result of GC-MS analysis of the methylated oil revealed 15 chemical components. The three major fatty components of the oil were oleic acid (58.88%), palmitic acid (26.16%) and glycerylmonooleate (5.27%). The anti-tubercular activity of the oil was attributed to the oleic acid and palmitic acid content. The oil could be a substitute for other oleic acid rich edible oil as well as a raw material for industrial production of oleic and palmitic acid derivatives and oleochemicals. The findings in this study could be exploited in new anti-tubercular drug research and drug design.