The study was carried out on essential oil of Artemisia herbaalba. CG/SM qualitative analysis of white wormwood essential oil allowed us to identify 99.61% of the total peak areas. The main one is Bicyclo [2.2.1] heptan-2-one, 1,7,7-trimethyl-, (1R)- (29.82%). Other main components are determined as 1,3-Cyclopentadiene, 1,2,5,5- tetramethyl- (15.58), Eucalyptol (6,51%) in the oil. On a biological level, evaluation of the essential oil revealed an interesting antimicrobial activity towards Escherichia coli, Staphylococcus aureus, Candida albicans, Fusarium oxysporum f. sp. ciceris(Chickpea Fusarium Wilt), Fusarium solani (Chickpea root-rot) and Globisporangium ultimum (Aleppo pine damping off and root-rot diseases).Results are also interesting for the pharmaceutical and agro-food industry fields using essential oil and this oil can be used in disease management against these soil-borne pathogens.