This study aims to investigate the major constituents of hexane fraction, of Calliandra haematocephala (Hassk.) leaves. In addition to, the hepatoprotective and antioxidant activities of the total alcohol extract against carbon tetrachloride (CCl4) -induced liver damage in vivo. C. haematocephala leaves were extracted with methanol, and hexane, fraction of methanol was separated and two major bands and were identified by GC/MS, MS, 1HNMR. Fatty acids were isolated, methylated and identified by GC/MS. In vivo the hepatoprotective and antioxidant activities of methanol extract against CCl4-induced liver injury was evaluated in rats based on the analysis of biochemical parameters and histopathological studies. The results illustrated that the analysis of hexane fraction led to isolation of lupeol and mixture of sterols. Four major fatty acids (palmitic, oleic, linoleic and linolenic,) were determined. Oral administration of the extract at doses 100 and 200 mg/kg bw. caused significant decrease in the levels of serum ALT, AST, GGT and bilirubin as well as albumin was significantly elevated. Moreover, the extract decreased MDA content and increased the levels of SOD, GSH and CAT compared with intoxicated rats. Also, creatinine and urea were improved as a result of the treatment of the extract. The hepatoprotective and antioxidant effect of the extract may be attributed to phenolics, flavonoids or the saponins content of the extract which may be acting as free radical scavenging effect, inhibiting lipidperoxidation and increasing antioxidant activities suggesting that the alcohol extract of C. haematocephala leaves has potential to be explored as valuable hepatoprotective and antioxidant .