A series of tri substituted imidazoles were synthesized in good yields by one pot three component condensation of benzil, aldehyde and ammonium acetate. The notable features of this methodology are simple procedure, cost effective and product purified by non- chromatographic techniques. The as-synthesized derivatives are characterized by IR, NMR and Mass spectroscopy. From the spectroscopic results the proposed structure of the imidazole derivatives was predicted. Moreover, its antimicrobial activity is also tested by disc diffusion technique.