The study was to develop a mouse model of non-insulin-dependent diabetes mellitus (NIDDM) that similar to human clinical pathogenesis. Fed three week-old C57BL/6J mice with chow enriched in sugar and fat for 3 weeks, then injected those mice with Streptozotocin (STZ, 125mg/kg) and continued feeding for 4 weeks. After 7 weeks high fat diets, the blood glucose concentration of mice in high-fat-STZ group reached the standard of NIDDM mouse.