Health care students are in permanent contact and handling with biological agents, and using jackets in inappropriate environments and can contaminate clothing, other students, the environment and the population. The present study aimed to verify the contamination rate of jackets of students investigating the situation of biosafety. The collect was held with sterile swab in the tissues, in an area of approximately 4 cm2 of the sleeves and of the front of the jackets of 100 students and the samples were plated on Petri containing Blood Agar for microbiological analysis. Microbial contamination was detected in 85% of the jackets; being 78,84% Pharmacy students, 95% Nursing, 94,44% Dentistry and 80% Medicine. There were observed the presence of Gram-negative and Grampositive cocci, bacilli, hemolytic bacteria. It concludes that the jackets are contamination vectors, requiring awareness from the students to the practice of Biosafety.