In present work A series of novel chalcones of 4- bromoacetophenone have been synthesized by using substituted aldehydes like 2-chlorobenzaldehyde, N-N dimethyl benzaldehyde and Isatin by regular Claisen condonsation reaction. The synthesized compounds were characterized by IR and H1NMR. The chalcones are bioactive in nature hence this synthesized compounds were evaluated for biological activity againsed the some gram positive and gram negative bacteria and againsed some fungus. These substituted chalcones show good inhibition activities.