Syzygium cumini (L.) Skeels is a native tree of China and the Antilles, cultivated in many countries, including Brazil, which the fruit is popularly known as jambolan or “widow earring”. Biological activitiessuch as antidiabetic (hypoglycemic agent), antidiarrheal, anti-inflammatory, antimicrobial and antioxidant are described in literature for this specie. Even though some studies have described the phytochemical profile of S. cumini, there are few studies on the biological activities evaluation of the atomized extract by spray drying.This study aims to assess cell viability by cytotoxicity assay and to evaluate the biological activities leishmanicide, antioxidant and antimicrobial of atomized aqueous extract of the Syzygium cumini (L.) Skells fruit. The atomized extract showed antioxidant activity, and showed no cell death at concentrations of 1μM, 10 μM and 100 μM, nor leishmanicideactivity at the concentrations tested. Regarding the antimicrobial activity, it was observed that the extract presents higher sensitivity to the microorganism S. flexneri. The results of the biological activity assay demonstrated that the drying process by spray dryingis a feasible and promising possibility to obtain intermediate products.