The macroalgae showed varied quantities of biochemical constituents like total carbohydrate, total protein and total lipid. Studies were conducted to evaluate biochemical composition of Spatoglossum asperum. Fatty acid, vitamins and minerals composition were carried out by using gas chromatography method and flame atomic absorption spectrophotometry method respectively. Among biochemical content total protein is present in high amount, 11.03 ± 0.06%, total carbohydrates is 6.55 ± 0.14% and total lipid is 3.14± 0.14%. Among the 7 identified fatty acid, Linoleic acid (29.41± 0.01%) and stearic acid (21.99 ± 0.01%) are found to be the major components. Among vitamins, vitamin C (15.60 ± 0.00 mg/100gm) and Niacin (10.86 ± 0.02 mg/100gm) are present in major quantities. Macro minerals such as Potassium (203.38 ± 0.02 mg/100gm), Sodium (145.50 ± 0.10 mg/100gm) and Calcium (134.51 ± 0.01mg/100gm) were identified in higher amount.