Grape rhizosheric soil (Nasik region) harbors vast microbial abundance and diversity. In the current study we report two species of bacteria and fungi and grown on various medium like PDB, King’s B, Jensen, SDA. Further biochemical, morphological and molecular studies on specific strains were carried out. Soil carbon, Electrical conductivity and pH were also estimated. The bacterial species are plant growth promoting microorganisms (PGMS) which are screened for their beneficial traits like Indole (IAA) production and Urease production capabilities. Species of pseudomonas and A. Niger are potentially responsible for P solublization, Azotobacter and Azotobacter produces IAA and GA which are plant growth promoters. Aspergillus spp. suppressed the growth of anthracnose and the fluorescent Pseudomonads shows the maximum inhibition zone for pathogen. The use of these chemical components as well as microbial reactions help in nutrient availability, growth and development of plants which results in more crop production, and they can be improved by manipulating the beneficial rhizosphere microorganisms.