The present study was carried out to screen insecticidal, anthelmintic, pancreatic amylase and pancreatic lipase inhibitory activity of 17 Streptomyces species (PO-01 to PO-16 and PO-18) isolated from soils of different places of Western Ghats of Shivamogga district, Karnataka, India. Insecticidal activity was assessed in terms of larvicidal effect against 2nd and 3rd instar larvae of Aedes aegypti. 2nd instar larvae were more susceptible to extracts when compared to 3rd instar larvae. Isolates PO-02, PO-08 and PO-11 showed marked larvicidal efficacy when compared to other isolates. Anthelmintic activity, in terms of time taken for paralysis and death of worms, was performed using adult Indian earthworm model. Two isolate namely PO-02 and PO-13 showed marked anthelmintic efficacy. Amylase inhibitory effect of solvent extracts was tested against pancreatic amylase. Isolates PO-13 and PO-14 displayed marked inhibitory effect when compared to other isolates. Inhibitory activity of solvent extracts against lipase activity was tested against lipase from chicken pancreas. Among isolates, two isolates namely PO-16 and PO- 01 showed high and least inhibitory activity against lipase activity respectively. In conclusion, Streptomyces species of Western Ghats of Shivamogga district are promising resources for development of insecticidal and anthelmintic agents and enzyme inhibitors. Further studies are to be carried out to isolate active principles from solvent extracts and to determine their bioactivities.