Exploring the use of bio-sorbents in controlling Chromium (VI) pollution in waste waters is one of the main areas of present research on pollution control methods. In the present work, barks and their ashes of Azadirachta indica, Syzygium cumini and Acacia arabica are found to have strong affinity towards Chromium (VI) ions. A thorough investigation has been made in optimizing the various physicochemical parameters such as equilibration time, pH and sorption dosage for the maximum extraction of Chromium (VI) from waste waters. % of extraction of Chromium(VI) is pH sensitive and it decrease with increase of pH and also depends on sorption concentration and time of equilibration. With these sorbents % of extractability is found to be more than 96.0% at optimum conditions extraction. Ten fold excess of common ions present in natural waters, viz., Ca2+, Mg2+ , Cu2, Zn2+ and Ni2 + , Nitrates, Chloride, Fluoride and Carbonate , have less interfered the % of extraction at the experimental conditions but Sulphates and Phosphates have showed some interference to some extent but, the extraction has never come down below 70.3%. Sorbent dosage and time needed for the maximum removal of Chromium (VI) is less for the barks ashes than with the raw bark powders. The applicability of the methodologies developed is tested with real effluent samples of industries and natural lake samples and found remarkably successful.